Human adipose derived stem cells (hASCs) have been seeded onto polymer microarrays that had been fabricated utilizing a wide range of acrylate monomers to find novel substrates that induced differentiation in direction of chondrocytes and osteoblasts.
Stream cytometric evaluation confirmed that each CD105 and CD49d optimistic hASCs elevated quickly with passage quantity on the lead polymers, whereas quantitative PCR evaluation confirmed that the substrate synthesized from methacryloxyethyltrimethyl ammonium chloride, N,N-diethylaminoethyl methacrylate and cyclohexyl methacrylate enhanced chondrogenesis and osteogenensis some Four and 25 occasions respectively when it comes to the expression of SOX9 and ALP in differentiated stem cells. These copolymers substrates thus have nice potential for utility within the purification, era and enlargement of outlined hASC’s and the managed differentiation of of cells for potential medical utility.
Roles of apoptotic chondrocyte-derived CXCL12 within the enhanced chondroclast recruitment following methotrexate and/or dexamethasone therapy
Intensive use of methotrexate (MTX) and/or dexamethasone (DEX) for treating childhood malignancies is understood to trigger chondrocyte apoptosis and progress plate dysfunction resulting in bone progress impairments. Nevertheless, mechanisms stay obscure and it’s unclear whether or not MTX and DEX mixture therapy might have additive results within the progress plate defects. On this research, important cell apoptosis was induced in mature ATDC5 chondrocytes after therapy for 48 h with 10-5 M MTX and/or 10-6 M DEX therapy. PCR array assays with handled cells plus messenger RNA and protein expression affirmation analyses recognized chemokine CXCL12 having essentially the most distinguished induction in every therapy group.
Conditioned medium from handled chondrocytes stimulated migration of RAW264.7 osteoclast precursor cells and formation of osteoclasts, and these stimulating results have been inhibited by the neutralizing antibody for CXCL12. Moreover, whereas MTX and DEX mixture therapy confirmed some additive results on apoptosis induction, it didn’t have additive or counteractive results on CXCL12 expression and its features in enhancing osteoclastic recruitment and formation.
In younger rats handled acutely with MTX, there was elevated expression of CXCL12 within the tibial progress plate, and extra resorbing chondroclasts have been discovered current on the border between the hypertrophic progress plate and metaphysis bone. Thus, the current research confirmed an affiliation between induced chondrocyte apoptosis and stimulated osteoclastic migration and formation following MTX and/or DEX therapy, which may very well be doubtlessly or not less than partially linked molecularly by CXCL12 induction. This discovering could contribute to an enhanced mechanistic understanding of bone progress impairments following MTX and/or DEX remedy.
Fraxetin inhibits interleukin-1β-induced apoptosis, irritation, and matrix degradation in chondrocytes and protects rat cartilage in vivo
Osteoarthritis (OA) is a illness characterised by degeneration of the joint complicated on account of cartilage destruction. Fraxetin, a broadly used and studied coumarin compound extracted from a standard Chinese language herb (Qin Pi), has proven anti-inflammatory and antioxidant properties, however its results on OA haven’t been studied. Within the current research, western blotting, immunofluorescence, and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) have been used to guage the results of fraxetin on IL-1β-induced apoptotic exercise, inflammatory responses, and catabolism in rat chondrocytes.
The outcomes confirmed that fraxetin prevented IL-1β-induced apoptosis of chondrocytes and inhibited inflammatory mediator launch by regulating the Toll-like receptor 4 (TLR4)/myeloid differentiation major response 88 (MyD88)/nuclear issue (NF)-κB pathway in chondrocytes. Moreover, fraxetin suppressed the upregulation of matrix metalloproteinase 13 (MMP13) and degradation of collagen II within the extracellular matrix (ECM).
Furthermore, the results of fraxetin in vivo have been assessed in a monosodium iodoacetate (MIA)-induced rat mannequin of OA utilizing hematoxylin and eosin (H&E) and Safranin O-fast inexperienced staining and magnetic resonance imaging (MRI). The outcomes confirmed that fraxetin protected the cartilage in opposition to destruction. In conclusion, fraxetin may very well be a possible therapeutic for OA.
Bioinspired Mineralization Utilizing Chondrocyte Membrane Nanofragments
Biomineralization includes complicated processes and interactions between natural and inorganic issues, that are managed partly by the cells. The goals of this research have been, first, to carry out a scientific and ultrastructural investigation of the preliminary mineral formation throughout secondary ossification middle of mouse femur primarily based on materials science and biology viewpoint, after which develop novel biomaterials for mineralization primarily based on the in vivo findings. First, we recognized the very preliminary mineral deposition at postnatal day 5 (P5) on the medial facet of femur epiphysis by nanocomputed tomography. Preliminary minerals have been discovered within the environment of hypertrophic chondrocytes.
Apparently, histological and immunohistochemical analyses confirmed that preliminary mineralization till P6 was primarily based on chondrocyte exercise solely, i.e., it occurred within the absence of osteoblasts. Furthermore, electron microscopy-based ultrastructural evaluation confirmed that cell-secreted matrix vesicles have been absent within the early steps of osteoblast-independent endochondral ossification.
As a substitute, chondrocyte membrane nanofragments have been discovered within the fibrous matrix surrounding the hypertrophic chondrocytes. EDS evaluation and electron diffraction research indicated that cell membrane nanofragments weren’t mineralized materials, and may very well be the nucleation website for the newly shaped calcospherites.
The phospholipids within the cell membrane nanofragments may very well be a supply of phosphate for subsequent calcium phosphate formation, which initially was amorphous, and later remodeled into apatite crystals. Lastly, synthetic cell nanofragments have been synthesized from ATDC5 chondrogenic cells, and in vitro assays confirmed that these nanofragments might promote mineral formation.
 Butoconazole nitrate |
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| B1902-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: Butoconazole nitrate |
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Butoconazole nitrate |
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| B1902-50 | ApexBio | 50 mg | EUR 128 |
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Description: Butoconazole nitrate |
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Butoconazole nitrate |
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| B1902-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Butoconazole nitrate |
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 Econazole nitrate |
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| B1937-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: Econazole nitrate |
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Econazole nitrate |
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| B1937-50 | ApexBio | 50 mg | EUR 128 |
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Description: Econazole nitrate |
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Econazole nitrate |
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| B1937-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Econazole nitrate |
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 Isoconazole nitrate |
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| B1956-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: Isoconazole nitrate |
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Isoconazole nitrate |
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| B1956-50 | ApexBio | 50 mg | EUR 128 |
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Description: Isoconazole nitrate |
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Isoconazole nitrate |
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| B1956-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Isoconazole nitrate |
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 Miconazole Nitrate |
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| B1978-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: Miconazole Nitrate |
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Miconazole Nitrate |
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| B1978-50 | ApexBio | 50 mg | EUR 128 |
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Description: Miconazole Nitrate |
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Miconazole Nitrate |
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| B1978-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Miconazole Nitrate |
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 Sulconazole Nitrate |
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| B2036-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 142 |
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Description: Sulconazole Nitrate is an imidazole derivative with broad-spectrum antifungal activity. |
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Sulconazole Nitrate |
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| B2036-50 | ApexBio | 50 mg | EUR 128 |
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Description: Sulconazole Nitrate is an imidazole derivative with broad-spectrum antifungal activity. |
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Sulconazole Nitrate |
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| B2036-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Sulconazole Nitrate is an imidazole derivative with broad-spectrum antifungal activity. |
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Econazole nitrate |
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| E001-100G | TOKU-E | 100 g | EUR 499 |
Econazole nitrate |
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| E001-25G | TOKU-E | 25 g | EUR 208 |
Econazole nitrate |
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| E001-5G | TOKU-E | 5 g | EUR 79 |
 Thiamine nitrate |
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| 20-abx180904 | Abbexa |
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Isoconazole nitrate |
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| 20-abx184134 | Abbexa |
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 Miconazole nitrate |
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| 20-abx184229 | Abbexa |
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) Oxiconazole (nitrate) |
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| C3418-1000 | ApexBio | 1 g | EUR 163 |
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Description: Oxiconazole is a new imidazole derivative with a broad fungistatic spectrum against the agents of human mycoses in vitro. Subinhibitory concentrations of oxiconazole inhibited synthesis of DNA and decreased synthesis of RNA, protein and carbohydrate to a lesser extent. |
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Oxiconazole (nitrate) |
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| C3418-25000 | ApexBio | 25 g | EUR 1185 |
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Description: Oxiconazole is a new imidazole derivative with a broad fungistatic spectrum against the agents of human mycoses in vitro. Subinhibitory concentrations of oxiconazole inhibited synthesis of DNA and decreased synthesis of RNA, protein and carbohydrate to a lesser extent. |
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Oxiconazole (nitrate) |
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| C3418-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 113 |
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Description: Oxiconazole is a new imidazole derivative with a broad fungistatic spectrum against the agents of human mycoses in vitro. Subinhibitory concentrations of oxiconazole inhibited synthesis of DNA and decreased synthesis of RNA, protein and carbohydrate to a lesser extent. |
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Oxiconazole (nitrate) |
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| C3418-5000 | ApexBio | 5 g | EUR 390 |
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Description: Oxiconazole is a new imidazole derivative with a broad fungistatic spectrum against the agents of human mycoses in vitro. Subinhibitory concentrations of oxiconazole inhibited synthesis of DNA and decreased synthesis of RNA, protein and carbohydrate to a lesser extent. |
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 Fenticonazole Nitrate |
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| A8432-10 | ApexBio | 10 mg | EUR 108 |
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Description: Fenticonazole Nitrate is an azole antifungal drug that inhibits CYP51A1 (14 ?-demethylase) and ergosterol production. It shows active effects against Malassezia furfur and Candida albicans in vivo. |
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Fenticonazole Nitrate |
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| A8432-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 122 |
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Description: Fenticonazole Nitrate is an azole antifungal drug that inhibits CYP51A1 (14 ?-demethylase) and ergosterol production. It shows active effects against Malassezia furfur and Candida albicans in vivo. |
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Fenticonazole Nitrate |
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| A8432-50 | ApexBio | 50 mg | EUR 270 |
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Description: Fenticonazole Nitrate is an azole antifungal drug that inhibits CYP51A1 (14 ?-demethylase) and ergosterol production. It shows active effects against Malassezia furfur and Candida albicans in vivo. |
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) Butoconazole (nitrate) |
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| HY-B0293 | MedChemExpress | 10mM/1mL | EUR 126 |
) Fenticonazole (Nitrate) |
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| HY-B0359 | MedChemExpress | 10mM/1mL | EUR 134 |
) Econazole (nitrate) |
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| HY-B0453 | MedChemExpress | 5g | EUR 160 |
) Miconazole (nitrate) |
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| HY-B0454A | MedChemExpress | 5g | EUR 160 |
) Sertaconazole (nitrate) |
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| HY-B0736A | MedChemExpress | 10mM/1mL | EUR 113 |
) Pilocarpine (nitrate) |
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| HY-B1006 | MedChemExpress | 100mg | EUR 133 |
 Oxiconazole nitrate |
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| HY-B1324 | MedChemExpress | 10mg | EUR 133 |
) Isoconazole (nitrate) |
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| HY-B1444 | MedChemExpress | 100mg | EUR 158 |
) Sulconazole (nitrate) |
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| HY-B1460A | MedChemExpress | 100mg | EUR 133 |
Thiamine nitrate |
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| HY-B2223 | MedChemExpress | 10mM/1mL | EUR 126 |
) Dehydrocorydaline (nitrate) |
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| HY-N4238 | MedChemExpress | 1mg | EUR 179 |
Thiamine nitrate |
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| GV4958-100G | Glentham Life Sciences | 100 g | EUR 122 |
Thiamine nitrate |
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| GV4958-25G | Glentham Life Sciences | 25 g | EUR 70 |
 Fenticonazole nitrate |
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| GP1083-100MG | Glentham Life Sciences | 100 mg | EUR 78 |
Fenticonazole nitrate |
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| GP1083-250MG | Glentham Life Sciences | 250 mg | EUR 118 |
Isoconazole nitrate |
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| GA9375-1G | Glentham Life Sciences | 1 g | EUR 62 |
 Sodium nitrate |
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| GK2648-100G | Glentham Life Sciences | 100 g | EUR 58 |
 Silver nitrate |
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| GK7224-100G | Glentham Life Sciences | 100 g | EUR 221 |
Silver nitrate |
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| GK7224-10G | Glentham Life Sciences | 10 g | EUR 70 |
Silver nitrate |
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| GK7224-250G | Glentham Life Sciences | 250 g | EUR 373 |
Silver nitrate |
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| GK7224-25G | Glentham Life Sciences | 25 g | EUR 94 |
Silver nitrate |
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| GK7224-50G | Glentham Life Sciences | 50 g | EUR 142 |
Silver nitrate |
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| GK7224-5G | Glentham Life Sciences | 5 g | EUR 56 |
 isa nitrate |
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| ISA31 | Consort | ea | EUR 91 |
 nitrate electrode |
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| ISE31B | Consort | ea | EUR 352 |
Miconazole nitrate |
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| M005-1G | TOKU-E | 1 g | EUR 62 |
Miconazole nitrate |
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| M005-5G | TOKU-E | 5 g | EUR 173 |
 NITRATE BROTH |
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| N14-106-10kg | Alphabiosciences | 10 kg | EUR 1428 |
NITRATE BROTH |
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| N14-106-2kg | Alphabiosciences | 2kg | EUR 350 |
NITRATE BROTH |
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| N14-106-500g | Alphabiosciences | 500 g | EUR 131 |
 NITRATE AGAR |
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| N14-107-10kg | Alphabiosciences | 10 kg | EUR 1303 |
NITRATE AGAR |
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| N14-107-2Kg | Alphabiosciences | 2 Kg | EUR 322 |
NITRATE AGAR |
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| N14-107-500g | Alphabiosciences | 500 g | EUR 124 |
 Dehydrocorydaline nitrate |
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| TBW01112 | ChemNorm | 20mg | Ask for price |
 Barium nitrate |
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| BB0119 | Bio Basic | 1Kg | EUR 123.08 |
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 Potassium nitrate |
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| PD0444 | Bio Basic | 500g | EUR 63.92 |
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Silver nitrate |
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| SB0479 | Bio Basic | 25g | EUR 142.22 |
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Sodium nitrate |
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| SD0484 | Bio Basic | 500g | EUR 61.31 |
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 Indole-3-carbinol |
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| B2839-1 | Biovision | 1 g | EUR 106 |
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Description: Indole-3-carbinol is an anticancer phytochemical in cruciferous vegetables. It is produced by the breakdown of the glucosinolate glucobrassicin by the action of enzyme myrosinase. It induces apoptosis in cancer cells. It inhibits NF-?B, Akt and MAPK signaling pathways. It inactivates a ubiquitin E3 ligase (WWP1), which restores the tumor suppressive activity of PTEN. |
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Indole-3-carbinol |
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| B2839-100 | Biovision | EUR 109 | |
Indole-3-carbinol |
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| B2839-5 | Biovision | 5 g | EUR 147 |
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Description: Indole-3-carbinol is an anticancer phytochemical in cruciferous vegetables. It is produced by the breakdown of the glucosinolate glucobrassicin by the action of enzyme myrosinase. It induces apoptosis in cancer cells. It inhibits NF-?B, Akt and MAPK signaling pathways. It inactivates a ubiquitin E3 ligase (WWP1), which restores the tumor suppressive activity of PTEN. |
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Indole-3-carbinol |
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| B2839-500 | Biovision | EUR 153 | |
Indole-3-carbinol |
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| B3667-100 | ApexBio | 100 mg | EUR 96 |
Indole-3-carbinol |
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| B3667-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
 Indole-6-Carboxaldehyde |
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| abx188736-5g | Abbexa | 5 g | EUR 314 |
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indole) 5-(Trifluoromethyl)indole |
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| 20-abx181189 | Abbexa |
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Indole-3-carbinol |
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| E1KS2313 | EnoGene | 100mg | EUR 247 |
Indole-3-carbinol |
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| HY-N0170 | MedChemExpress | 1g | EUR 147 |
 Indole-3-carboxaldehyde |
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| GK9020-100G | Glentham Life Sciences | 100 g | EUR 150 |
Indole-3-carboxaldehyde |
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| GK9020-10G | Glentham Life Sciences | 10 g | EUR 58 |
Indole-3-carboxaldehyde |
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| GK9020-25G | Glentham Life Sciences | 25 g | EUR 78 |
Indole-3-carboxaldehyde |
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| GK9020-5G | Glentham Life Sciences | 5 g | EUR 48 |
Indole-3-carbinol |
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| GK5424-1G | Glentham Life Sciences | 1 g | EUR 46 |
Indole-3-carbinol |
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| GK5424-25G | Glentham Life Sciences | 25 g | EUR 158 |
Indole-3-carbinol |
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| GK5424-5G | Glentham Life Sciences | 5 g | EUR 70 |
 Indole-3-acetamide |
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| HY-W016784 | MedChemExpress | 100mg | EUR 108 |
Indole-3-carbinol |
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| I034-1G | TOKU-E | 1 g | EUR 63 |
Indole-3-carbinol |
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| I034-25G | TOKU-E | 25 g | EUR 199 |
Indole-3-carbinol |
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| I034-5G | TOKU-E | 5 g | EUR 77 |
 Indole-3-glyoxylamide |
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| TBP03437 | ChemNorm | unit | Ask for price |
) Econazole Nitrate (powder) |
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| 50R-R14827 | Fitzgerald | 50 mg | EUR 133 |
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Description: Econazole Nitrate chemical reference substance |
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 Aluminium nitrate nonahydrate |
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| abx184820-100g | Abbexa | 100 g | EUR 244 |
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 Rhodium nitrate solution |
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| 20-abx186014 | Abbexa |
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 Nitrate Standard, 200UL |
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| C086-200UL | Arbor Assays | 200UL | EUR 123 |
 Nitrate Reductase, 1EA |
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| C088-1EA | Arbor Assays | 1EA | EUR 87 |
 nitrate) (4-Acetamidocyclohexyl) nitrate |
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| HY-100295 | MedChemExpress | 10mg | EUR 1153 |
) Cycloguanil (D6 Nitrate) |
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| HY-12784S1 | MedChemExpress | 1mg | EUR 271 |
 Zinc nitrate hexahydrate |
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| GX0843-100G | Glentham Life Sciences | 100 g | EUR 44 |
Zinc nitrate hexahydrate |
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| GX0843-1KG | Glentham Life Sciences | 1 kg | EUR 98 |
Zinc nitrate hexahydrate |
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| GX0843-250G | Glentham Life Sciences | 250 g | EUR 54 |
Zinc nitrate hexahydrate |
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| GX0843-500G | Glentham Life Sciences | 500 g | EUR 70 |
Zinc nitrate hexahydrate |
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| GX0843-5KG | Glentham Life Sciences | 5 kg | EUR 233 |
 Barium nitrate, 99% |
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| GX5821-100G | Glentham Life Sciences | 100 g | EUR 50 |
Barium nitrate, 99% |
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| GX5821-1KG | Glentham Life Sciences | 1 kg | EUR 159 |
Barium nitrate, 99% |
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| GX5821-250G | Glentham Life Sciences | 250 g | EUR 70 |
Barium nitrate, 99% |
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| GX5821-500G | Glentham Life Sciences | 500 g | EUR 102 |
 L-Anserine · nitrate |
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| G-4555.0100 | Bachem | 100.0mg | EUR 212 |
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Description: Sum Formula: C10H16N4O3·HNO3; CAS# [10030-52-1] |
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L-Anserine · nitrate |
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| G-4555.0250 | Bachem | 250.0mg | EUR 393 |
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Description: Sum Formula: C10H16N4O3·HNO3; CAS# [10030-52-1] |
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 Miconazole nitrate salt |
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| GA3642-1G | Glentham Life Sciences | 1 g | EUR 54 |
Miconazole nitrate salt |
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| GA3642-25G | Glentham Life Sciences | 25 g | EUR 229 |
Miconazole nitrate salt |
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| GA3642-5G | Glentham Life Sciences | 5 g | EUR 86 |
 Econazole nitrate salt |
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| GA5978-100G | Glentham Life Sciences | 100 g | EUR 289 |
Econazole nitrate salt |
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| GA5978-25G | Glentham Life Sciences | 25 g | EUR 134 |
Econazole nitrate salt |
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| GA5978-5G | Glentham Life Sciences | 5 g | EUR 62 |
 Calcium nitrate tetrahydrate |
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| GE9273-10KG | Glentham Life Sciences | 10 kg | EUR 166 |
Calcium nitrate tetrahydrate |
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| GE9273-1KG | Glentham Life Sciences | 1 kg | EUR 54 |
Calcium nitrate tetrahydrate |
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| GE9273-500G | Glentham Life Sciences | 500 g | EUR 46 |
Calcium nitrate tetrahydrate |
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| GE9273-5KG | Glentham Life Sciences | 5 kg | EUR 110 |
 Magnesium nitrate hexahydrate |
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| GK0164-1KG | Glentham Life Sciences | 1 kg | EUR 66 |
Magnesium nitrate hexahydrate |
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| GK0164-250G | Glentham Life Sciences | 250 g | EUR 44 |
Magnesium nitrate hexahydrate |
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| GK0164-500G | Glentham Life Sciences | 500 g | EUR 54 |
Magnesium nitrate hexahydrate |
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| GK0164-5KG | Glentham Life Sciences | 5 kg | EUR 178 |
 Potassium nitrate, 99% |
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| GK1121-1KG | Glentham Life Sciences | 1 kg | EUR 78 |
Potassium nitrate, 99% |
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| GK1121-250G | Glentham Life Sciences | 250 g | EUR 50 |
Potassium nitrate, 99% |
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| GK1121-500G | Glentham Life Sciences | 500 g | EUR 59 |
Potassium nitrate, 99% |
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| GK1121-5KG | Glentham Life Sciences | 5 kg | EUR 181 |
 2-Ethylhexyl nitrate |
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| GK6026-100ML | Glentham Life Sciences | 100 ml | EUR 50 |
2-Ethylhexyl nitrate |
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| GK6026-1L | Glentham Life Sciences | 1 l | EUR 134 |
2-Ethylhexyl nitrate |
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| GK6026-500ML | Glentham Life Sciences | 500 ml | EUR 86 |
 calibration solution nitrate |
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| ISC31 | Consort | ea | EUR 91 |
 Pseudopalmatine methyl nitrate |
|||
| TBZ1841 | ChemNorm | unit | Ask for price |
Calcium nitrate tetrahydrate |
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| CB0258 | Bio Basic | 500g | EUR 64.79 |
|
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Magnesium nitrate hexahydrate |
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| MB0586 | Bio Basic | 500g | EUR 67.4 |
|
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 Zinc nitrate, hexahydrate |
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| ZB1004 | Bio Basic | 500g | EUR 96.98 |
|
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 5-Hydroxy-Indole [BSA] |
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| DAG3257 | Creative Diagnostics | 0.05mg | EUR 650 |
 QuantiChrom Indole Assay Kit |
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| DIND-100 | BioAssay Systems | 100 | EUR 210 |
|
Description: determination in biological samples by colorimetric (565nm) method. Procedure: Under 5 min. Kit size: 100 tests. Detection limit: 3 to 100 µM. Shelf life: 6 months. Shipping: ambient temp; storage: 4°C. |
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 7-Bromo-1H-Indole |
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| abx188562-25g | Abbexa | 25 g | EUR 453 |
|
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 5-Bromo-1H-indole |
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| 20-abx181194 | Abbexa |
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 3-Indole glyoxylic acid |
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| 20-abx185788 | Abbexa |
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 5-Methylsulfanyl-1H-Indole |
|||
| 20-abx186340 | Abbexa |
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 Methyl indole-4-carboxylate |
|||
| abx182565-25g | Abbexa | 25 g | EUR 314 |
|
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Taken collectively, these outcomes indicated that cell membrane nanofragments have been the nucleation website for mineral formation, and will doubtlessly be used as materials for manipulation of biomineralization.
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