Mechanistic insights into AMPK-SIRT3 positive feedback loop-mediated chondrocyte mitochondrial quality control in osteoarthritis pathogenesis

Osteoarthritis (OA) is a significant reason behind incapacity within the aged inhabitants and represents a big public well being downside and socioeconomic burden worldwide. Nonetheless, no disease-modifying therapeutics are at present out there for OA resulting from an inadequate understanding of the pathogenesis of this incapacity. As a novel cell kind in cartilage, chondrocytes are important for cartilage homeostasis and play a essential position in OA pathogenesis. Mitochondria are necessary metabolic facilities in chondrocytes and contribute to cell survival, and mitochondrial high quality management (MQC) is an rising mechanism for sustaining cell homeostasis.

An growing variety of latest research have demonstrated that dysregulation of the important thing processes of chondrocyte MQC, which contain mitochondrial redox, biogenesis, dynamics, and mitophagy, is related to OA pathogenesis and could be regulated by the chondroprotective molecules 5′ adenosine monophosphate-activated protein kinase (AMPK) and sirtuin 3 (SIRT3).

Furthermore, AMPK and SIRT3 regulate one another, and their expression and exercise are all the time constant in chondrocytes, which counsel the existence of an AMPK-SIRT3 optimistic suggestions loop (PFL). Though the exact mechanisms usually are not absolutely elucidated and want additional validation, the present literature signifies that this AMPK-SIRT3 PFL regulates OA improvement and development, not less than partially by mediating chondrocyte MQC. Due to this fact, understanding the mechanisms of AMPK-SIRT3 PFL-mediated chondrocyte MQC in OA pathogenesis would possibly yield new concepts and potential targets for subsequent analysis on the OA pathomechanism and therapeutics.

SOX9 retains development plates and articular cartilage wholesome by inhibiting chondrocyte dedifferentiation/osteoblastic redifferentiation

Cartilage is important all through vertebrate life. It begins growing in embryos when osteochondroprogenitor cells decide to chondrogenesis, activate a pancartilaginous program to type cartilaginous skeletal primordia, and in addition embrace a growth-plate program to drive skeletal development or an articular program to construct everlasting joint cartilage. Varied types of cartilage malformation and degeneration illnesses afflict people, however underlying mechanisms are nonetheless incompletely understood and therapy choices suboptimal.

The transcription issue SOX9 is required for embryonic chondrogenesis, however its postnatal roles stay unclear, regardless of proof that it’s down-regulated in osteoarthritis and heterozygously inactivated in campomelic dysplasia, a extreme skeletal dysplasia characterised postnatally by small stature and kyphoscoliosis. Utilizing conditional knockout mice and high-throughput sequencing assays, we present right here that SOX9 is required postnatally to stop growth-plate closure and preosteoarthritic deterioration of articular cartilage.

Its deficiency prompts growth-plate chondrocytes in any respect levels to swiftly attain a terminal/dedifferentiated stage marked by expression of chondrocyte-specific (Mgp) and progenitor-specific (Nt5e and Sox4) genes. Up-regulation of osteogenic genes (Runx2, Sp7, and Postn) and overt osteoblastogenesis shortly ensue. SOX9 deficiency doesn’t perturb the articular program, besides in load-bearing areas, the place it additionally provokes chondrocyte-to-osteoblast conversion by way of a progenitor stage. Pathway analyses help roles for SOX9 in controlling TGFβ and BMP signaling actions throughout this cell lineage transition.

Altogether, these findings deepen our present understanding of the mobile and molecular mechanisms that particularly guarantee lifelong growth-plate and articular cartilage vigor by figuring out osteogenic plasticity of growth-plate and articular chondrocytes and a SOX9-countered chondrocyte dedifferentiation/osteoblast redifferentiation course of.

Allogeneic adipose-derived mesenchymal stem cells promote the expression of chondrocyte redifferentiation markers and retard the development of knee osteoarthritis in rabbits

Osteoarthritis (OA) is a progressively degenerative illness of joints. In vitro tradition of chondrocytes leads to dedifferentiation, which is characterised by the event of fibroblast phenotypes, diminished potential to supply cartilage extracellular matrix (ECM) and improve the expression of molecular markers Col1a1, Col10a1 and Runx2. Redifferentiation of chondrocytes is indicated by elevated expression of the molecular markers Col2a1, Aggrecan and Sox9.

Within the present research, we investigated the results of allogeneic rabbit adipose-derived mesenchymal stem cells (ADSCs) on articular chondrocytes, and explored the therapeutic impact of ADSCs on broken articular cartilage at totally different levels in a rabbit OA mannequin. In vitro, the proliferation and migration of major articular chondrocytes have been enhanced by cocultured rabbit ADSCs, and the expression of redifferentiation markers in chondrocytes cocultured with ADSCs was elevated at each the mRNA and protein ranges, whereas the expression of dedifferentiation markers was decreased. 

In vivo, the rabbit mannequin of OA was established by anterior cruciate ligament transection (ACLT) with full medial meniscectomy (MMx). Two weeks after surgical procedure, ADSCs have been used for OA rabbit therapy. Intra-articular injection of ADSCs progressively alleviated articular cartilage destruction, decreased Osteoarthritis Analysis Society Worldwide (OARSI) and Mankin scores, and diminished MMP13 expression at totally different levels within the rabbit mannequin of OA. Throughout the experiment, allogeneic ADSCs didn’t trigger any antagonistic occasions. The present research demonstrates the results and molecular mechanisms of ADSCs on articular chondrocytes and gives a good reference for scientific OA therapy with mesenchymal stem cells (MSCs) derived from adipose tissue.

Bioprinting of human nasoseptal chondrocytes-laden collagen hydrogel for cartilage tissue engineering

Pores and skin most cancers sufferers typically have tumorigenic lesions on their noses. Surgical resection of the lesions typically leads to nasal cartilage elimination. Cartilage grafts taken from different anatomical websites are used for the surgical reconstruction of the nasal cartilage, however donor-site morbidity is a standard downside. Autologous tissue-engineered nasal cartilage grafts can mitigate the issue, however commercially out there scaffolds outline the form and sizes of the engineered grafts throughout tissue fabrication.

Furthermore, the engineered grafts endure from the inhomogeneous distribution of the purposeful matrix of cartilage. Advances in 3D bioprinting know-how supply the chance to engineer cartilages with customizable dimensions and anatomically formed configurations with out the inhomogeneous distribution of cartilage matrix. Right here, we report the constancy of Freeform Reversible Embedding of Suspended Hydrogel (FRESH) bioprinting as a technique to generate customizable and homogenously distributed purposeful cartilage matrix engineered nasal cartilage. Utilizing FRESH and in vitro chondrogenesis, we now have fabricated tissue-engineered nasal cartilage from combining bovine kind I collagen hydrogel and human nasoseptal chondrocytes. The engineered nasal cartilage constructs displayed molecular, biochemical and histological traits akin to native human nasal cartilage.

Curcumin Supplementation Enhances Bone Marrow Mesenchymal Stem Cells to Promote the Anabolism of Articular Chondrocytes and Cartilage Restore

Mesenchymal stem cells derived from bone marrows (BMSCs) and curcumin derived from turmeric have been used for osteoarthritis (OA) therapy, respectively. We invested the results of curcumin supplementation for BMSC therapeutic results. In vitro, rat BMSCs have been recognized by dual-immunofluorescent staining of CD44 and CD90, and circulation cytometry. Major articular chondrocytes have been recognized by toluidine blue staining and immunofluorescent staining of Col2a1.

EdU incorporation, migration assay, real-time quantitative polymerase chain response, and Western blot analyses have been carried out to guage the alterations of chondrocytes cocultured with BMSCs. In vivo, the rat mannequin of OA was established by monoiodoacetic acid. After intra-articular injection of allogeneic BMSCs, articular cartilage injury and OA development have been evaluated by histological staining, and Osteoarthritis Analysis Society Worldwide and Mankin rating analysis.

Though curcumin alone didn’t enhance cell viability of major articular chondrocytes, it promoted proliferation and migration of chondrocytes when cocultured with BMSCs. In the meantime, the expression of anabolic genes in chondrocytes was remarkably elevated each at mRNA and protein ranges. In OA rats, curcumin and BMSCs cooperated to significantly promote articular cartilage restore and retard OA development.

Macconkey Agar 2Kg
212122	Scientific Laboratory Supplies	EACH	EUR 430.8

Macconkey Agar Ii 500gm
212306	Scientific Laboratory Supplies	EACH	EUR 200.4

Sorbitol MacConkey Agar
MED1340	Scientific Laboratory Supplies	EACH	EUR 85.2

MacConkey Agar No.3
MED1256	Scientific Laboratory Supplies	PK10	EUR 10.8

MacConkey Agar No.3
MED1358	Scientific Laboratory Supplies	EACH	EUR 79.2

Macconkey Agar Base 500gm
281810	Scientific Laboratory Supplies	EACH	EUR 210

MacConkey Agar with Salt
MED1450	Scientific Laboratory Supplies	EACH	EUR 103.2

Macconkey Agar 90mm Plate
215197	Scientific Laboratory Supplies	PK20	EUR 52.8

MACCONKEY SORBITOL AGAR
M13-110-10kg	Alphabiosciences	10 kg	EUR 1122

MACCONKEY SORBITOL AGAR
M13-110-2kg	Alphabiosciences	2kg	EUR 290.4

MACCONKEY SORBITOL AGAR
M13-110-500g	Alphabiosciences	500 g	EUR 122.4

MacConkey Agar without Salt
MED1198	Scientific Laboratory Supplies	PK10	EUR 6

MacConkey Agar w/o Salt & CV
MED1342	Scientific Laboratory Supplies	EACH	EUR 98.4

MACCONKEY AGAR W/O CRYSTAL VIOLET
M13-108-10kg	Alphabiosciences	10 kg	Ask for price

MACCONKEY AGAR W/O CRYSTAL VIOLET
M13-108-2kg	Alphabiosciences	2kg	Ask for price

MACCONKEY AGAR W/O CRYSTAL VIOLET
M13-108-500g	Alphabiosciences	500 g	Ask for price

MACCONKEY BROTH
M13-111-10kg	Alphabiosciences	10 kg	EUR 1196.4

MACCONKEY BROTH
M13-111-2Kg	Alphabiosciences	2 Kg	EUR 307.2

MACCONKEY BROTH
M13-111-500g	Alphabiosciences	500 g	EUR 127.2

MacConkey Broth (USP)
MED1662	Scientific Laboratory Supplies	500G	EUR 67.2

Agarose, medium EEO
GE4750-100G	Glentham Life Sciences	100 g	EUR 199.2

Agarose, medium EEO
GE4750-500G	Glentham Life Sciences	500 g	EUR 514.8

Agarose, medium EEO
GE4750-100	Glentham Life Sciences	100	EUR 134.4

Agarose, medium EEO
GE4750-25	Glentham Life Sciences	25	EUR 53.8

Agarose, medium EEO
GE4750-500	Glentham Life Sciences	500	EUR 395.5

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 126

A Medium
DJ1018	Bio Basic	100g	EUR 101.76

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1894.8

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 458.4

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 168

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 976.8

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 259.2

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 114

OF Medium
MED1664	Scientific Laboratory Supplies	500G	EUR 79.2

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

Ec Medium 500gm
231430	Scientific Laboratory Supplies	EACH	EUR 176.4

TCBS Medium
MED1188	Scientific Laboratory Supplies	PK10	EUR 9.6

M9CA Medium
SD7025	Bio Basic	250g	EUR 86.1

Sob Medium 500gm
244310	Scientific Laboratory Supplies	EACH	EUR 261.6

DNase Medium
MED1196	Scientific Laboratory Supplies	PK10	EUR 10.8

TYGPN Medium
SD7032	Bio Basic	250g	EUR 84.01

Heller's Medium
CP014-010	GenDepot	10X1L	EUR 118.8

Heller's Medium
CP014-500	GenDepot	50L	EUR 151.2

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price

Mounting Medium
4300	Virostat	3 ml	EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 123.6

COLIFORM MEDIUM
C03-127-10kg	Alphabiosciences	10 kg	EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg	Alphabiosciences	2kg	EUR 392.4

COLIFORM MEDIUM
C03-127-500g	Alphabiosciences	500 g	EUR 150

Hoagland's Medium
CP015-010	GenDepot	10X1L	EUR 118.8

Hoagland's Medium
CP015-500	GenDepot	50L	EUR 151.2

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price

RPMI 1640 Medium
abx098879-500ml	Abbexa	500 ml	EUR 210

A-1 MEDIUM
A01-116-10kg	Alphabiosciences	10 kg	EUR 1240.8

A-1 MEDIUM
A01-116-2Kg	Alphabiosciences	2 Kg	EUR 316.8

A-1 MEDIUM
A01-116-500g	Alphabiosciences	500 g	EUR 129.6

MCDB 131 Medium
CM034-050	GenDepot	500ml	EUR 102

MCDB 131 Medium
CM034-300	GenDepot	6x500ml	EUR 198

MCDB 131 Medium
CM034-310	GenDepot	10x500ml	EUR 262.8

MCDB 131 Medium
CM034-320	GenDepot	20x500ml	EUR 408

MCDB 131 Medium
CM034-350	GenDepot	50x500ml	EUR 702

Medium 199/BCDB 105
C0012-02	Addexbio	RT 500 mL Bottle	EUR 181.2

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004	Allele Biotech	1 liter	Ask for price

Isp Medium 2 500gm
277010	Scientific Laboratory Supplies	EACH	EUR 356.4

Isp Medium 4 500gm
277210	Scientific Laboratory Supplies	EACH	EUR 340.8

BPS Medium 1
79259	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway)

BPS Medium 7
79260	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits.

BPS Medium 8
79385	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379).

Thaw Medium 4
60181-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 4
60181-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 5
60182-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 5
60182-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 6
60183-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 6
60183-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 2
60184-1	BPS Bioscience	100 ml	EUR 150
Description: This liquid Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's Jurkat-based cell lines, including• PD-1/NFAT Reporter-Jurkat cell #60535• NFAT Reporter (Luc) - Jurkat Cell Line #60621• NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540• ADCC Bioassay Effector Cell V variant (High Affinity) #60541Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 2
60184-2	BPS Bioscience	500 ml	EUR 675
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D__x000D_• PD-1/NFAT Reporter-Jurkat cell #60535_x000D__x000D_• NFAT Reporter (Luc) - Jurkat Cell Line #60621_x000D__x000D_• NF-ĸB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651_x000D__x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540_x000D__x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) #60541_x000D__x000D_Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 7
60185-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623

Thaw Medium 7
60185-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including • Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623

Thaw Medium 3
60186-1	BPS Bioscience	100 ml	EUR 150
Description: This liquid Cell Thaw Medium is optimized for thawing and plating BPS Bioscience's CHO cell-based recombinant cell lines, including• TCR Activator - CHO Recombinant Cell line #60539 • TCR activator / PD-L1 - CHO Recombinant Cell line #60536 It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. Note: once cells are well established, switch to an appropriate cell growth medium.

Thaw Medium 3
60186-2	BPS Bioscience	500 ml	EUR 645
Description: This liquid thaw medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including_x000D__x000D_• TCR Activator - CHO Recombinant Cell line #60539 _x000D_• TCR activator / PD-L1 - CHO Recombinant Cell line #60536 _x000D__x000D_• CD27 CHO-K1 Stable Recombiant Cell Line #60624 _x000D__x000D_• PD-L1 - CHO Recombinant Cell Line #60543 _x000D__x000D_• NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line #60622_x000D_• CD137L (4-1BBL) CHO-K1 Recombinant Cell Line #60523_x000D__x000D_It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. _x000D_Note: once cells are well established, switch to an appropriate cell growth medium._x000D_

Thaw Medium 1
60187-1	BPS Bioscience	100 ml	EUR 155
Description: This liquid HEK293 Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's HEK293 recombinant cell lines.

Thaw Medium 1
60187-2	BPS Bioscience	500 ml	EUR 695
Description: This liquid Thaw Medium is optimized for thawing and plating BPS Bioscience's HEK293 recombinant cell lines.

10mL S.O.C. Medium
46-003-CR	Scientific Laboratory Supplies	PK10	EUR 122.4

Thaw Medium 12
78074-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 12
78074-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 14
78397-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 14
78397-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 8
79652-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 8
79652-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 9
79665-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D_• CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 9
79665-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, using • CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 10
79704-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 10
79704-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 11
79976-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 11
79976-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 15
78412-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15
78412-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 16
78647-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 16
78647-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Of Basal Medium 500gm
268820	Scientific Laboratory Supplies	EACH	EUR 224.4

Medium 199 (Mod) Powder
90-050PB	Scientific Laboratory Supplies	EACH	EUR 93.48

Assay Medium 1B
79617-1	BPS Bioscience	100 ml	EUR 135
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 1B
79617-2	BPS Bioscience	500 ml	EUR 675
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 2B
79619-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 2B
79619-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Due to this fact, curcumin supplementation enhanced the BMSC operate for the proliferation and migration of articular chondrocytes, and anabolic gene expression of extracellular matrix in articular chondrocytes in vitro, and the technology of articular cartilage in vivo. Our research make clear the potential scientific software of curcumin cooperated with BMSCs in cartilage restore for OA therapy.