Hoaglands Medium; Ohne Stickstoff

Switchgrass  (  Panicum virgatum  L.) is a warm-season rhizome-like  perennial  grass that can tolerate various abiotic stresses while providing relatively high biomass, and is recognized as a leading biofuel feedstock  for  peripheral areas. Nitrogen (N) is crucial for  switchgrass growth and development  , and its tolerance to low N supply and high N utilization efficiency are very important for its production under poor conditions.

 Large-scale cultivation of switchgrass on marginal land could be an effective approach to solving the problem of raw material supply for energy from biomass. This study used a  hydroponic experiment to evaluate the effect of N-deficiency on switchgrass seedlings.

Three N treatments (0, 0.15, and 1.50 mmol L Hoagland’s solution) and six cultivars were used, three from each  ecotype  (upland and lowland). The results showed that biomass, leaf area, root surface area,  net photosynthesis  and total chlorophyll content decreased significantly under N-poor treatments compared to those in full strength Hoagland broth. However, once established, all plants survived extreme N-stress (0 mmol L ) and were partially productive. The Kanlow variety performed best of the six varieties under stress. Significant interactions between stress treatment and cultivars showed that breeding high-yielding cultivars with superior performance under N deficiency is justified. Lowland  outperformed upland ecotypes under stress, suggesting that lowland cultivars can survive and be productive under a broader range of stress conditions However, given the better adaptability of lowland ecotypes to hydroponic cultivation, further studies are needed.

introduction

Increasing energy security and mitigating climate change are the two main drivers that have pushed renewable energy generation to the top of the global agenda. They encourage agronomic production of biomass to meet renewable bioenergy needs.

  • Perennial  grasses are attractive as biomass sources because they can meet the agronomic, environmental, and social requirements for successful use as energy crops.
  • Perennial rhizome-like grass is an ideal biofuel crop because of its agronomically desirable traits of broad climate tolerance , rapid growth rates, and relatively high yields.  Additionally, perennial grasses have low nutrient requirements due to nutrient recycling through their rhizome systems . They are also rarely attacked by pests and can therefore be made with little or no pesticides .
  • Given these unique advantages, interest in using biofuel crops for energy production is increasing. However, since China cannot afford to produce energy from biomass on its arable land, biofuel cultivation, in order to be competitive with conventional energy sources and avoid crowding out food crops, will likely be relegated to less productive soils and receive minimal water supply receive.
  • Fertilizers and PesticidesTherefore, peripheral areas can play an important role in the production of energy from biomass. It is estimated that the amount of marginal land that could be used for biofuel production in China is nearly 110 million hectares, of which about 45 million hectares would support economic operations. Abiotic pressures such as nutrient deficiencies, drought and high salinity in these areas are common factors limiting the production of biofuel crops.

Under environmental stress such as nitrogen (N) deficiency, which will be a major limiting factor for growing biofuel crops in Northwest and North China, plants show different adaptations at morphological, biochemical, molecular and physiological levels. It is imperative to update our knowledge of the tolerance of biofuel crops to various nutrient deficiency conditions to be expanded to allow continuous industrialization of biomass in peripheral areas. Efficient production of bioenergy from such marginal areas requires selection of the most stress-tolerant grass species. Biofuel crops are being screened or bred for superior traits and genetically modified for enhanced abiotic stress tolerance traits that will expand their acreage  [6] . Accordingly, it is desirable to assess the responses of promising biofuel crops to N-deficiency stress and to identify cultivars best suited for  biomass production  under N-deficiency conditions.

Switchgrass  (  Panicum virgatum  L.) is a warm season rhizome forming perennial  C4  grass native to the North American tall grass prairies.

Because of its broad adaptability, rapid growth rate, ability to grow on low-productivity soils, and ability to function as a component in a multipurpose cropping system, it is a leading dedicated biofuel  feedstock candidate  in the United States. It reacts strongly to N fertilizers and is often drought tolerant. It can effectively sequester carbon in the soil and provides excellent cover for wildlife. With many beneficial traits as energy crops, the Department of Energy’s Bioenergy Feedstock Development Program (BFDP) decided to focus research on a model crop system and focus research resources on switchgrass to quickly realize its maximum yield as a  biomass  crop .

There are two distinct  ecotypes  of switchgrass:  lowland  tetraploid  and upland octoploid.

The lowland tetraploid ecotype originates primarily from the southern extent of the native range and the upland octoploid primarily from its mid-to-northern extent. Several dozen cultivated cultivars of each ecotype are commercially available, most of which are high-yielding selections from native populations. The species shows large differences in performance in relation to environmental variables, although lowland ecotypes typically produce larger yields than upland ecotypes.

Previous studies have mainly focused on the response of switchgrass biomass to N-nutrient suppl. The effect of N-deficiency on switchgrass has not been extensively studied, particularly in hydroponically cultivated seedlings, and knowledge of the effects of different levels of N-deficiency on agronomic traits  , photosynthetic parameters, and chlorophyll content in switchgrass is limited.

Hoagland's Medium; Without Nitrogen

CP016-010 GenDepot 10X1L 118.8 EUR

Hoagland's Medium; Without Nitrogen

CP016-500 GenDepot 50L 151.2 EUR

Hoagland's Medium

CP015-010 GenDepot 10X1L 118.8 EUR

Hoagland's Medium

CP015-500 GenDepot 50L 151.2 EUR

EMM Growth Medium without Nitrogen

G055-500G EWC Diagnostics 1 unit 63.29 EUR

Yeast Nitrogen Base Medium without Amino Acids

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Hoagland & Knop Medium;w/ Agar;w/o Vitamins

PT126-10X1L EWC Diagnostics 1 unit 13.67 EUR

Hoagland & Knop Medium;w/ Agar;w/o Vitamins

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Hoagland & Knop Medium;w/ Agar;w/o Vitamins

PT126-5L EWC Diagnostics 1 unit 5.95 EUR

Murashige and Skoog, Without nitrogen

CP034-010 GenDepot 10X1L 121.2 EUR

Murashige and Skoog, Without nitrogen

CP034-500 GenDepot 50L 151.2 EUR

Yeast Nitrogen Base Medium

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Norris Glucose Nitrogen Free Medium

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Bismuth sulphite Medium (without

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Dextrose Tryptone Medium (without

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Sabouraud Dextrose Medium (without

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MacConkey Medium (without Membrane

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Pseudomonas Medium (without Membrane

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Pseudomonas Medium (without Membrane

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EMM Growth Medium without Dextrose

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ECD Medium (without Membrane Filter).

MF009-50PT EWC Diagnostics 1 unit 41.98 EUR

Slanetz and Bartley Medium (without

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#Orange Serum Medium (without Membrane

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Teepol Medium (without Membrane Filter).

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Teepol Medium (without Membrane Filter).

MF015-50PT EWC Diagnostics 1 unit 41.98 EUR

#ECC Selective Medium (without Membrane

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KSOM Mouse Embryo Medium without AA

TBS8071-50ML Tribioscience 50mL 41 EUR

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R-2A Medium (Without membrane filter)

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MCDB 131 Medium, Without L-Glutamin, Without Glucose and No Phenol Red

CM134-050 GenDepot 500ml 35 EUR

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M-Endo Medium (without Membrane Filter).

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Stuart Medium without Methylene blue wit

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500mL PF Medium without L-glutam - PK6

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MEMα (without nucleoside) Complete Medium

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Potato Dextrose Medium w/ TTC (Without M

MF039-20PT EWC Diagnostics 1 unit 25.5 EUR

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CHO|ONE E, Expression Medium, without Insulin, without L-Glutamine, with Pluronic - 1000 ml

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MEMalpha Complete Medium (without nucleoside, with 10%FBS)

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Nanobacteria Removal Medium (MEMalpha, without nucleoside)

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Nanobacteria Removal Medium (MEMalpha, without nucleoside)

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Nanobacteria Removal Medium (MEMalpha, without nucleoside)

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FUNCTIONALITY/VIABILITY MEDIUM, CMRL 1066 (WITHOUT GLUCOSE)

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ProCHO 4 Protein-free Medium, without Phenol Red, FFM, 1L

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VeroPrime, Chemically Defined Medium, without L-Glutamine - 500 ml

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ProCHO 5 Protein-free Medium, without Phenol Red, FFM, 1 L

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Nanobacteria Removal Complete Medium (MEMα, without nucleoside)

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Nanobacteria Removal Complete Medium (MEMα, without nucleoside)

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EGM-2 Endothelial Medium SingleQuot Kit (without Basal media)

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RPMI 1640, powder medium, with L-Glutamine, without Sodium Bicarbonate - 1 Bottle

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Nanobacteria Removal Complete Medium (MEMα, without nucleoside, with 10%FBS)

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MEM with EBSS, powder medium, with L-Glutamine, without Sodium Bicarbonate - 1 Bottle

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Hoagland No. 2 Basal Salt Mixture

TS1094-5L EWC Diagnostics 1 unit 4.52 EUR

Hoagland No. 2 Basal Salt Mixture w/o

TS1117-10X1L EWC Diagnostics 1 unit 8.28 EUR

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Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 20%FBS)

MBS2570272-100mL MyBiosource 100mL 135 EUR

Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 20%FBS)

MBS2570272-5x100mL MyBiosource 5x100mL 600 EUR

Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 10%FBS)

MBS2570353-100mL MyBiosource 100mL 125 EUR

Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 10%FBS)

MBS2570353-5x100mL MyBiosource 5x100mL 545 EUR

Hoagland No. 2 Basal Salt Mixture (Modif

TS1105-10X1L EWC Diagnostics 1 unit 10.65 EUR

Hoagland No. 2 Basal Salt Mixture (Modif

TS1105-5L EWC Diagnostics 1 unit 4.61 EUR

Endothelial Basal Medium MCDB 120 (Powder)
Base powder without supplements

MBS652968-100L MyBiosource 100L 1550 EUR

Endothelial Basal Medium MCDB 120 (Powder)
Base powder without supplements

MBS652968-10L MyBiosource 10L 335 EUR

Endothelial Basal Medium MCDB 120 (Powder)
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MBS652968-25L MyBiosource 25L 600 EUR

Endothelial Basal Medium MCDB 120 (Powder)
Base powder without supplements

MBS652968-50L MyBiosource 50L 975 EUR

Endothelial Basal Medium MCDB 120 (Powder)
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MBS652968-5x100L MyBiosource 5x100L 5925 EUR

HOAGLAND MODIFIED BASAL SALT MIXTURE

H353 PhytoTechnology Laboratories 100L 31.63 EUR

10 L MEDIUM 199 (MOD.), POWDER WITH EARLE'S SALTS WITHOUT SODIUM BICARBONATE, L-GLUTAMINE, AND PHENOL RED

90-050-PB CORNING 10 L/pk 93.6 EUR

HEK|ONE S, chemically defined medium for stable expression, without L-Glut, with Growth Hormones

CAHEKS-1000ML Westburg each 166.73 EUR

HEK|ONE T, chemically defined medium for transient expression, without L-Glut, with Growth Hormones

CAHEKT-1000ML Westburg each 171.05 EUR

Corning 96 Well White Polystyrene Medium Bind Stripwell Microplate 25 per Bag without Lid Nonsterile - PK100

3913 Scientific Laboratory Supplies PK100 965.25 EUR

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3914 Scientific Laboratory Supplies PK100 965.25 EUR

Corning 96 Well Black Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile - PK100

2482 Scientific Laboratory Supplies PK100 1075.95 EUR

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2484 Scientific Laboratory Supplies PK100 1121.85 EUR

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

MBS652833-100g MyBiosource 100g 195 EUR

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

MBS652833-1kg MyBiosource 1kg 780 EUR

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

MBS652833-25kg MyBiosource 2.5kg 1020 EUR

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

MBS652833-500g MyBiosource 500g 455 EUR

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

MBS652833-5x25kg MyBiosource 5x2.5kg 4310 EUR

ISA NITROGEN OXIDE

ISA52 Consort ea 135 EUR

*Yeast Nitrogen Base

M139-100G EWC Diagnostics 1 unit 33.1 EUR

*Yeast Nitrogen Base

M139-500G EWC Diagnostics 1 unit 133.53 EUR

Nitrogen dioxide 99.5%

N08955 Pfaltz & Bauer 400G 2032.96 EUR

YEAST NITROGEN BASE

Y25-104-10kg Alphabiosciences 10 kg 6818.4 EUR

YEAST NITROGEN BASE

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YEAST NITROGEN BASE

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Nitrogen trifluoride 99.99%

N08990 Pfaltz & Bauer 598G 3330.62 EUR

NITROGEN OXIDES ELECTRODE

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Urea Nitrogen Standard, 1ML

C089-1ML Arbor Assays 1ML 144 EUR

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NtcA | Global nitrogen regulator

AS12-1873 Agrisera AB 200 µg 413 EUR

IceOff Liquid Nitrogen Drizzler

API-IceOff-01 MiTeGen each 324 EUR

IceOff Liquid Nitrogen Drizzler

TE MiTeGen 1 UNIT 324 EUR

Soil Nitrate Nitrogen Assay Kit

SH0104 Jiaxing Korain Biotech Ltd (BT Labs) 50T/48Sample 260 EUR

Cryo Kit for liquid nitrogen

IPD9600-CK Benchmark Scientific each 1027.94 EUR

Automatic Liquid Nitrogen Filler

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Plant Nitrate Nitrogen Assay Kit

SH0101 Jiaxing Korain Biotech Ltd (BT Labs) 50T/48Sample 280 EUR

Nitrogen Std 0.1% w/w - 100ML

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Yeast Nitrogen Base 100gm - EACH

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Soil Ammonium Nitrogen Assay Kit

SH0238 Jiaxing Korain Biotech Ltd (BT Labs) 50T/48Sample 350 EUR

Blood Urea Nitrogen Serum Samples

MBS173601-1Sample MyBiosource 1Sample 285 EUR

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MBS173601-5x1Sample MyBiosource 5x1Sample 1100 EUR

Plant Ammonium Nitrogen Assay Kit

SH0245 Jiaxing Korain Biotech Ltd (BT Labs) 50T/48Sample 280 EUR

Blood Urea Nitrogen Detection Kit

SKT-213-192 Stressmarq 2 plates of 96 wells 227.25 EUR

Rat urea nitrogen (BUN) ELISA Kit

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Nitrogen Total 0.0 to25.0mg/l - EACH

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LN20 Liquid Nitrogen Generator - EACH

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SLS6158 Scientific Laboratory Supplies EACH 88678.8 EUR

Total Nitrogen Std 1000ppm (N) - 500ML

TN1H Scientific Laboratory Supplies 500ML 468.45 EUR

Blood Urea Nitrogen (BUN) Assay Kit

abx090684-100l Abbexa 100 µl 225 EUR

Blood Urea Nitrogen (BUN) Assay Kit

abx090684-100tests Abbexa 100 tests 284.4 EUR

Blood Urea Nitrogen (BUN) Assay Kit

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Rat Blood Urea Nitrogen ELISA kit

E01A10513 BlueGene 96T 700 EUR

Rat Blood Urea Nitrogen ELISA kit

E02B0697-192T BlueGene 192 tests 1524 EUR

Rat Blood Urea Nitrogen ELISA kit

E02B0697-48 BlueGene 1 plate of 48 wells 624 EUR

Rat Blood Urea Nitrogen ELISA kit

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Dog Blood Urea Nitrogen ELISA kit

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Dog Blood Urea Nitrogen ELISA kit

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Dog Blood Urea Nitrogen ELISA kit

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Pig Blood Urea Nitrogen ELISA kit

E07B0697-192T BlueGene 192 tests 1524 EUR

Pig Blood Urea Nitrogen ELISA kit

E07B0697-48 BlueGene 1 plate of 48 wells 624 EUR

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LN20AC Liquid Nitrogen Generator - EACH

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SLS6152 Scientific Laboratory Supplies EACH 73256.4 EUR

LN65AC Liquid Nitrogen Generator - EACH

SLS6156 Scientific Laboratory Supplies EACH 96390 EUR

Side wall Manual Nitrogen Tap - EACH

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Goat Blood Urea Nitrogen ELISA kit

E01A45425 BlueGene 96T 700 EUR

Goat Blood Urea Nitrogen ELISA kit

E06B0697-192T BlueGene 192 tests 1524 EUR

Goat Blood Urea Nitrogen ELISA kit

E06B0697-48 BlueGene 1 plate of 48 wells 624 EUR

Goat Blood Urea Nitrogen ELISA kit

E06B0697-96 BlueGene 1 plate of 96 wells 822 EUR

The aim of this study was to evaluate the performance and reproductive potential of six cultivars of the two ecotypes in response to N-deficiency stress and provide some theoretical basis for growing relatively high-yielding switchgrass on soils with low fertility and for breeding on high ones N content to create efficiency use.

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