Switchgrass ( Panicum virgatum L.) is a warm-season rhizome-like perennial grass that can tolerate various abiotic stresses while providing relatively high biomass, and is recognized as a leading biofuel feedstock for peripheral areas. Nitrogen (N) is crucial for switchgrass growth and development , and its tolerance to low N supply and high N utilization efficiency are very important for its production under poor conditions.
Large-scale cultivation of switchgrass on marginal land could be an effective approach to solving the problem of raw material supply for energy from biomass. This study used a hydroponic experiment to evaluate the effect of N-deficiency on switchgrass seedlings.
Three N treatments (0, 0.15, and 1.50 mmol L Hoagland’s solution) and six cultivars were used, three from each ecotype (upland and lowland). The results showed that biomass, leaf area, root surface area, net photosynthesis and total chlorophyll content decreased significantly under N-poor treatments compared to those in full strength Hoagland broth. However, once established, all plants survived extreme N-stress (0 mmol L ) and were partially productive. The Kanlow variety performed best of the six varieties under stress. Significant interactions between stress treatment and cultivars showed that breeding high-yielding cultivars with superior performance under N deficiency is justified. Lowland outperformed upland ecotypes under stress, suggesting that lowland cultivars can survive and be productive under a broader range of stress conditions . However, given the better adaptability of lowland ecotypes to hydroponic cultivation, further studies are needed.
introduction
Increasing energy security and mitigating climate change are the two main drivers that have pushed renewable energy generation to the top of the global agenda. They encourage agronomic production of biomass to meet renewable bioenergy needs.
- Perennial grasses are attractive as biomass sources because they can meet the agronomic, environmental, and social requirements for successful use as energy crops.
- Perennial rhizome-like grass is an ideal biofuel crop because of its agronomically desirable traits of broad climate tolerance , rapid growth rates, and relatively high yields. Additionally, perennial grasses have low nutrient requirements due to nutrient recycling through their rhizome systems . They are also rarely attacked by pests and can therefore be made with little or no pesticides .
- Given these unique advantages, interest in using biofuel crops for energy production is increasing. However, since China cannot afford to produce energy from biomass on its arable land, biofuel cultivation, in order to be competitive with conventional energy sources and avoid crowding out food crops, will likely be relegated to less productive soils and receive minimal water supply receive.
- Fertilizers and Pesticides. Therefore, peripheral areas can play an important role in the production of energy from biomass. It is estimated that the amount of marginal land that could be used for biofuel production in China is nearly 110 million hectares, of which about 45 million hectares would support economic operations. Abiotic pressures such as nutrient deficiencies, drought and high salinity in these areas are common factors limiting the production of biofuel crops.
Under environmental stress such as nitrogen (N) deficiency, which will be a major limiting factor for growing biofuel crops in Northwest and North China, plants show different adaptations at morphological, biochemical, molecular and physiological levels. It is imperative to update our knowledge of the tolerance of biofuel crops to various nutrient deficiency conditions to be expanded to allow continuous industrialization of biomass in peripheral areas. Efficient production of bioenergy from such marginal areas requires selection of the most stress-tolerant grass species. Biofuel crops are being screened or bred for superior traits and genetically modified for enhanced abiotic stress tolerance traits that will expand their acreage [6] . Accordingly, it is desirable to assess the responses of promising biofuel crops to N-deficiency stress and to identify cultivars best suited for biomass production under N-deficiency conditions.
Switchgrass ( Panicum virgatum L.) is a warm season rhizome forming perennial C4 grass native to the North American tall grass prairies.
Because of its broad adaptability, rapid growth rate, ability to grow on low-productivity soils, and ability to function as a component in a multipurpose cropping system, it is a leading dedicated biofuel feedstock candidate in the United States. It reacts strongly to N fertilizers and is often drought tolerant. It can effectively sequester carbon in the soil and provides excellent cover for wildlife. With many beneficial traits as energy crops, the Department of Energy’s Bioenergy Feedstock Development Program (BFDP) decided to focus research on a model crop system and focus research resources on switchgrass to quickly realize its maximum yield as a biomass crop .
There are two distinct ecotypes of switchgrass: lowland tetraploid and upland octoploid.
The lowland tetraploid ecotype originates primarily from the southern extent of the native range and the upland octoploid primarily from its mid-to-northern extent. Several dozen cultivated cultivars of each ecotype are commercially available, most of which are high-yielding selections from native populations. The species shows large differences in performance in relation to environmental variables, although lowland ecotypes typically produce larger yields than upland ecotypes.
Previous studies have mainly focused on the response of switchgrass biomass to N-nutrient suppl. The effect of N-deficiency on switchgrass has not been extensively studied, particularly in hydroponically cultivated seedlings, and knowledge of the effects of different levels of N-deficiency on agronomic traits , photosynthetic parameters, and chlorophyll content in switchgrass is limited.
Hoagland's Medium; Without Nitrogen
CP016-010
GenDepot
10X1L
118.8 EUR
Hoagland's Medium; Without Nitrogen
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151.2 EUR
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32.77 EUR
Hoagland Medium, Nitrogen-Free
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55.37 EUR
Hoagland Medium, Nitrogen-Free
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GenDepot
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Yeast Nitrogen Base Medium without Amino Acids
HY-157347A
MedChemExpress
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Murashige and Skoog, Without nitrogen
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Yeast Nitrogen Base Medium
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MedChemExpress
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Medium 199 medium with 20mM HEPES, without glutamine
BS.F 0665
Bio&SELL
500 mL
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Murashige and Skoog (MS) Modified Medium w/o Nitrogen
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Murashige and Skoog (MS) Modified Medium w/o Nitrogen
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55.37 EUR
Medium 199 Earle's, without glutamine
BS.F 0615
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500 mL
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TERRESTRIAL ORCHID MEDIUM WITHOUT AGAR
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MCBD 153 basal medium, without glutamine
BS.F 8105
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MCDB 131 Medium, Without L-Glutamin, Without Glucose and No Phenol Red
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500mL PF Medium without L-glutam - PK6
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L-15 Leibovitz liquid medium, without glutamine
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MIAMI MEDIUM #1A CULTURE MEDIUM (WITH HUMAN
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MEMα Complete Medium (without nucleoside, with 20%FBS)
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SILAC RPMI-1640 Medium(without glucose, phenol red)
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MEMalpha Complete Medium (without nucleoside, with 10%FBS)
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160 EUR
MEMalpha Complete Medium (without nucleoside, with 20%FBS)
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Mycoplasma Removal Medium (MEMα, without nucleoside)
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500 mL
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Nanobacteria Removal Medium (MEMα, without nucleoside)
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148 EUR
Nanobacteria Removal Medium (MEMα, without nucleoside)
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Nanobacteria Removal Medium (MEMα, without nucleoside)
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Mycoplasma Removal Medium (MEMalpha, without nucleoside)
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450 EUR
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500 mL
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Nanobacteria Removal Medium (MEMalpha, without nucleoside)
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100mL
100 EUR
Nanobacteria Removal Medium (MEMalpha, without nucleoside)
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190 EUR
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175.2 EUR
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BS.F 0465
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500 mL
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Nanobacteria Removal Complete Medium (MEMα, without nucleoside)
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Elabscience Biotech
100mL
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Nanobacteria Removal Complete Medium (MEMα, without nucleoside)
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Grace's Insect TC Medium without L-Gutamin - Please inquire about availability
BS2.12G07J
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Nanobacteria Removal Complete Medium (MEMα, without nucleoside, with 10%FBS)
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100 mL
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Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 20%FBS)
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Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 20%FBS)
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Nanobacteria Removal Complete Medium (MEMalpha, without nucleoside, with 10%FBS)
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Endothelial Basal Medium MCDB 120 (Powder)
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195 EUR
Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)
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Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)
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1020 EUR
Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)
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500g
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135 EUR
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400G
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YEAST NITROGEN BASE
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YEAST NITROGEN BASE
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500 g
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Urea Nitrogen Standard, 1ML
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144 EUR
Urea Nitrogen Standard, 250UL
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85 EUR
Storage Liquid Nitrogen Tank
1-SLN100
Lab Expo
1 Each
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Storage Liquid Nitrogen Tank
10-SLN108
Lab Expo
1 Each
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Storage Liquid Nitrogen Tank
10-SLN109
Lab Expo
1 Each
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Storage Liquid Nitrogen Tank
10-SLN110
Lab Expo
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Storage Liquid Nitrogen Tank
10-SLN111
Lab Expo
1 Each
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NtcA | Global nitrogen regulator
AS12-1873
Agrisera AB
200 µg
413 EUR
Transport Liquid Nitrogen Tank
10-TNT100
Lab Expo
1 Each
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Williams' E-Medium, without phenol red
BS.F 1125
Bio&SELL
500 mL
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IceOff Liquid Nitrogen Drizzler
API-IceOff-01
MiTeGen
each
324 EUR
IceOff Liquid Nitrogen Drizzler
TE
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1 UNIT
324 EUR
Laboratory Liquid Nitrogen Tank
120-LNT106
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1 Each
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Laboratory Liquid Nitrogen Tank
147-LNT107
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Soil Nitrate Nitrogen Assay Kit
SH0104
Jiaxing Korain Biotech Ltd (BT Labs)
50T/48Sample
260 EUR
Cryo Kit for liquid nitrogen
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1027.94 EUR
Automatic Liquid Nitrogen Filler
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3850 EUR
Plant Nitrate Nitrogen Assay Kit
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Soil Ammonium Nitrogen Assay Kit
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Blood Urea Nitrogen Serum Samples
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285 EUR
Blood Urea Nitrogen Serum Samples
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1100 EUR
Blood Urea Nitrogen Detection Kit
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227.25 EUR
Plant Ammonium Nitrogen Assay Kit
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280 EUR
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Rat urea nitrogen (BUN) ELISA Kit
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48T
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Rat urea nitrogen (BUN) ELISA Kit
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96T
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LN20 Liquid Nitrogen Generator - EACH
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53978.4 EUR
LN30 Liquid Nitrogen Generator - EACH
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69400.8 EUR
LN65 Liquid Nitrogen Generator - EACH
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500ML
468.45 EUR
Rat Blood Urea Nitrogen ELISA kit
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700 EUR
Blood Urea Nitrogen (BUN) Assay Kit
abx090684-100l
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100 µl
225 EUR
Blood Urea Nitrogen (BUN) Assay Kit
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284.4 EUR
Blood Urea Nitrogen (BUN) Assay Kit
abx090684-1ml
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1 ml
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Blood Urea Nitrogen (BUN) Assay Kit
abx090684-200l
Abbexa
200 µl
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Rat Blood Urea Nitrogen ELISA kit
E02B0697-192T
BlueGene
192 tests
1524 EUR
Rat Blood Urea Nitrogen ELISA kit
E02B0697-48
BlueGene
1 plate of 48 wells
624 EUR
Rat Blood Urea Nitrogen ELISA kit
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280 EUR
Rat Blood Urea Nitrogen ELISA kit
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822 EUR
Rat Blood Urea Nitrogen ELISA kit
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405 EUR
Pig Blood Urea Nitrogen ELISA kit
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1524 EUR
Pig Blood Urea Nitrogen ELISA kit
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624 EUR
Pig Blood Urea Nitrogen ELISA kit
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822 EUR
Dog Blood Urea Nitrogen ELISA kit
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1524 EUR
Dog Blood Urea Nitrogen ELISA kit
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624 EUR
Dog Blood Urea Nitrogen ELISA kit
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822 EUR
Human urea nitrogen (BUN) ELISA Kit
CK-bio-24125-48T
Shanghai Coon Koon Biotech
48T
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Human urea nitrogen (BUN) ELISA Kit
CK-bio-24125-96T
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96T
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LN20AC Liquid Nitrogen Generator - EACH
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79203.15 EUR
LN30AC Liquid Nitrogen Generator - EACH
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LN65AC Liquid Nitrogen Generator - EACH
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Goat Blood Urea Nitrogen ELISA kit
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700 EUR
Goat Blood Urea Nitrogen ELISA kit
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1524 EUR
Goat Blood Urea Nitrogen ELISA kit
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624 EUR
Goat Blood Urea Nitrogen ELISA kit
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280 EUR
Goat Blood Urea Nitrogen ELISA kit
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822 EUR
Goat Blood Urea Nitrogen ELISA kit
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Side wall Manual Nitrogen Tap - EACH
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Nitrogen test 0.5 to 15mg/L - PK25
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223.26 EUR
TRITON2 Liquid Nitrogen Generator - EACH
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45241.98 EUR
Mouse Blood Urea Nitrogen ELISA kit
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700 EUR
Human Blood Urea Nitrogen ELISA kit
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Mouse Blood Urea Nitrogen ELISA Kit
DEIABL-M3
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704 EUR
Sheep Blood Urea Nitrogen ELISA kit
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280 EUR
Sheep Blood Urea Nitrogen ELISA kit
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Human Blood Urea Nitrogen ELISA kit
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192 tests
1524 EUR
Human Blood Urea Nitrogen ELISA kit
E01B0697-48
BlueGene
1 plate of 48 wells
624 EUR
Human Blood Urea Nitrogen ELISA kit
E01B0697-48wellsplate
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48 wells plate
280 EUR
Human Blood Urea Nitrogen ELISA kit
E01B0697-96
BlueGene
1 plate of 96 wells
822 EUR
Human Blood Urea Nitrogen ELISA kit
E01B0697-96wellsplate
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96 wells plate
405 EUR
Mouse Blood Urea Nitrogen ELISA kit
E03B0697-192T
BlueGene
192 tests
1524 EUR
Mouse Blood Urea Nitrogen ELISA kit
E03B0697-48
BlueGene
1 plate of 48 wells
624 EUR
Mouse Blood Urea Nitrogen ELISA kit
E03B0697-48wellsplate
BlueGene
48 wells plate
280 EUR
Mouse Blood Urea Nitrogen ELISA kit
E03B0697-96
BlueGene
1 plate of 96 wells
822 EUR
Mouse Blood Urea Nitrogen ELISA kit
E03B0697-96wellsplate
BlueGene
96 wells plate
405 EUR
Sheep Blood Urea Nitrogen ELISA kit
E01A97718
BlueGene
96T
700 EUR
NG1-0 nitrogen gas generator - EACH
SLS6010
Scientific Laboratory Supplies
EACH
6940.08 EUR
NG1-1 nitrogen gas generator - EACH
SLS6012
Scientific Laboratory Supplies
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7229.43 EUR
NG3-0 nitrogen gas generator - EACH
SLS6014
Scientific Laboratory Supplies
EACH
8386.85 EUR
NG3-1 nitrogen gas generator - EACH
SLS6016
Scientific Laboratory Supplies
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9447.32 EUR
NG5-0 nitrogen gas generator - EACH
SLS6018
Scientific Laboratory Supplies
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8773.14 EUR
NG5-1 nitrogen gas generator - EACH
SLS6020
Scientific Laboratory Supplies
EACH
9832.15 EUR
NG6-0 nitrogen gas generator - EACH
SLS6022
Scientific Laboratory Supplies
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11566.8 EUR
NG6-1 nitrogen gas generator - EACH
SLS6024
Scientific Laboratory Supplies
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15422.4 EUR
Automatic Nitrogen Generator BGEN-601
BGEN-601
Biolab Scientific Ltd.
Each
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Automatic Nitrogen Generator BGEN-602
BGEN-602
Biolab Scientific Ltd.
Each
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Automatic Nitrogen Generator BGEN-603
BGEN-603
Biolab Scientific Ltd.
Each
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Monkey Blood Urea Nitrogen ELISA kit
E01A71572
BlueGene
96T
700 EUR
Canine Blood Urea Nitrogen ELISA kit
E01A62859
BlueGene
96T
700 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E01A27993
BlueGene
96T
700 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E04B0697-192T
BlueGene
192 tests
1524 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E04B0697-48
BlueGene
1 plate of 48 wells
624 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E04B0697-48wellsplate
BlueGene
48 wells plate
280 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E04B0697-96
BlueGene
1 plate of 96 wells
822 EUR
Rabbit Blood Urea Nitrogen ELISA kit
E04B0697-96wellsplate
BlueGene
96 wells plate
405 EUR
Monkey Blood Urea Nitrogen ELISA kit
E09B0697-192T
BlueGene
192 tests
1524 EUR
The aim of this study was to evaluate the performance and reproductive potential of six cultivars of the two ecotypes in response to N-deficiency stress and provide some theoretical basis for growing relatively high-yielding switchgrass on soils with low fertility and for breeding on high ones N content to create efficiency use.

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