Osteoarthritis is characterised by cartilage loss ensuing from the activation of chondrocytes related to a synovial irritation. Activated chondrocytes promote an elevated secretion of matrix proteases and proinflammatory cytokines resulting in cartilage breakdown. Since pure merchandise possess anti-inflammatory properties, we investigated the direct impact of Rubus idaeus extracts (RIE) in chondrocyte metabolism and cartilage loss. The impact of RIE in chondrocyte metabolism was analyzed in murine main chondrocytes and cartilage explants.
We additionally assessed the contribution of RIE in an irritation surroundings by culturing mice main chondrocytes with the supernatant of Uncooked 264.7 macrophage-like cells primed with RIE. In main chondrocytes, RIE diminished chondrocyte hypertrophy (Col10), whereas growing the expression of catabolic genes (Mmp-3, Mmp-13) and decreasing anabolic genes (Col2a1, Acan).
In cartilage explants, Rubus idaeus prevented the lack of proteoglycan (14.84 ± 3.07% lack of proteoglycans with IL1 alone vs. 3.03 ± 1.86% with IL1 and 100 µg/mL of RIE), in addition to the NITEGE neoepitope expression. RIE alone lowered the expression of Il1 and Il6 in macrophages, with out adjustments in Tnf and Cox2 expression. The secretome of macrophages pre-treated with RIE and transferred to chondrocytes decreases the gene and protein expression of Mmp-3 and Cox2. In conclusion, these knowledge counsel that RIE could defend from chondrocyte catabolism and cartilage loss in inflammatory circumstances. Additional evaluations are want earlier than contemplating RIE as a candidate for the therapy for osteoarthritis.
AKAP2 overexpression modulates progress plate chondrocyte capabilities by way of ERK1/2 signaling
In our earlier examine, the mutation c.2645A > C (p. E882A) was discovered within the A-Kinase Anchoring Protein 2 (AKAP2) gene, which performs an vital position in regulating the event of the skeletal system; nonetheless, the precise impact of AKAP2 on chondrocyte proliferation and differentiation and the potential mechanism are nonetheless not clear. Within the current examine, we investigated the impact of AKAP2 in vitro. We efficiently remoted human progress plate chondrocytes (GPCs) from progress plate cartilage tissues and recognized GPCs by aggrecan expression and movement cytometric evaluation.
AKAP2 overexpression considerably promoted GPC proliferation, enhanced GPC differentiation, and promoted extracellular matrix (ECM) synthesis, whereas AKAP2 silencing exerted the alternative results on GPCs. AKAP2 overexpression elevated, whereas AKAP2 silencing decreased, the protein ranges of p-extracellular regulated protein kinases (ERK)1/2.
Extra importantly, the promotive results of AKAP2 overexpression on GPC proliferation, differentiation, and ECM synthesis have been considerably reversed by the ERK1/2 signaling antagonist U0126, suggesting that AKAP2 enhances GPC capabilities by way of ERK1/2 signaling. In conclusion, we reveal AKAP2 overexpression-induced enhancement of GPC capabilities by way of ERK1/2 signaling. Contemplating the important position of GPC capabilities in adolescent idiopathic scoliosis (AIS) pathogenesis, the appliance of AKAP2 concentrating on in AIS therapy must be investigated in future research.
Ameliorative Results of Loganin on Arthritis in Chondrocytes and Destabilization of the Medial Meniscus-Induced Animal Mannequin
Arthritis is a typical inflammatory illness that causes ache, stiffness, and joint swelling. Right here, we investigated the ameliorative results of loganin on arthritis in vitro and in vivo. A single bioactive compound was fractionated and remoted from Cornus officinalis (CO) extract to display for anti-arthritic results. A single element, loganin, was recognized as a candidate.
The CO extract and loganin inhibited the expression of things related to cartilage degradation, resembling cyclooxygenase-2 (COX-2), matrix metalloproteinase 3 (MMP-3), and matrix metalloproteinase 13 (MMP-13), in interukin-1 beta (IL-1β)-induced chondrocyte irritation. As well as, prostaglandin and collagenase ranges have been lowered following therapy of IL-1β-induced chondrocytes with loganin. Within the destabilization of the medial meniscus (DMM)-induced mouse mannequin, loganin administration attenuated cartilage degeneration by inhibiting COX-2, MMP-3, and MMP-13. Transverse micro-CT photos revealed that loganin lowered DMM-induced osteophyte formation. These outcomes point out that loganin has protecting results in DMM-induced mice.
SIRT1 immediately prompts autophagy in human chondrocytes
Osteoarthritis (OA) is the most typical type of arthritis worldwide with no efficient therapy. Ageing is the first threat issue for OA. We sought to research if there’s a distinct and useful convergence of ageing-related mechanisms SIRT1 and autophagy in chondrocytes. Our outcomes present that, ranges of SIRT1 are decreased in human regular aged and OA cartilage in contrast with younger cartilage.
Furthermore, silencing SIRT1 in chondrocytes result in decreased expression of chondrogenic markers however didn’t alter the expression of catabolic proteases. In distinction, activation of SIRT1 elevated autophagy in chondrocytes by the deacetylation of lysine residues on essential autophagy proteins (Beclin1, ATG5, ATG7, LC3). This activation was proven to be mTOR/ULK1 impartial. Our outcomes point out that upkeep of autophagy in chondrocytes by SIRT1 is important for preserving cartilage integrity all through life and due to this fact is a goal for drug intervention to guard in opposition to OA.
Molecular and Mobile Results of Chemical Chaperone-TUDCA on ER-Burdened NHAC-kn Human Articular Chondrocytes Cultured in Normoxic and Hypoxic Circumstances
Osteoarthritis (OA) is taken into account some of the frequent arthritic illnesses characterised by progressive degradation and irregular reworking of articular cartilage. Potential therapeutics for OA purpose at restoring correct chondrocyte functioning and inhibiting apoptosis. Earlier research have demonstrated that tauroursodeoxycholic acid (TUDCA) confirmed anti-inflammatory and anti-apoptotic exercise in lots of fashions of varied illnesses, appearing primarily by way of alleviation of endoplasmic reticulum (ER) stress. Nonetheless, little is thought about cytoprotective results of TUDCA on chondrocyte cells.
The current examine was designed to judge potential results of TUDCA on interleukin-1β (IL-1β) and tunicamycin (TNC)-stimulated NHAC-kn chondrocytes cultured in normoxic and hypoxic circumstances. Our outcomes confirmed that TUDCA alleviated ER stress in TNC-treated chondrocytes, as demonstrated by lowered CHOP expression; nonetheless, it was not efficient sufficient to stop apoptosis of NHAC-kn cells in both normoxia nor hypoxia.
Cellufine A-500 |
675980335 |
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10 lt |
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19-611-51 |
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19-865-51 |
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19-865-55 |
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19-845-15 |
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EUR 439.2 |
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19-845-51 |
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5 x 1 ml |
EUR 439.2 |
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195-15 |
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1 x 5 ml |
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195-51 |
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675982335 |
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675983335 |
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20-900 |
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100 ml |
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20-900-51 |
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20-900-55 |
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20-901 |
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20-902 |
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21-300-51 |
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21-300-55 |
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21-600-15 |
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21-600-51 |
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19-711-55 |
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21-000-51 |
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21-000-55 |
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21-900-11 |
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21-900-15 |
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21-100-51 |
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21-100-55 |
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MC Cellufine Etclean S |
201-15 |
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1 x 5 ml |
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MC Cellufine Etclean S |
201-51 |
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20-700-51 |
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5 x 1 ml |
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20-700-55 |
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5 x 5 ml |
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200-15 |
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1 x 5 ml |
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200-51 |
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MC Cellufine MAX AminoButyl |
21-500-15 |
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1 x 5 ml |
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21-500-51 |
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20-300-51 |
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20-400-51 |
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20-400-55 |
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20-500-51 |
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20-500-55 |
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20-600-51 |
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20-600-55 |
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20300-55 |
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MC Cellufine MAX Phenyl LS |
20-800-51 |
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5 x 1 ml |
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MC Cellufine MAX Phenyl LS |
20-800-55 |
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5 x 5 ml |
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MC Cellufine MAX DexS-Vir |
21-800-15 |
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MC Cellufine MAX DexS-Vir |
21-800-51 |
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Cellufine A-800 |
673980335 |
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10 lt |
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Cellufine A-200 |
676-980-327 |
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100 ml |
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Cellufine A-200 |
676980335 |
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683-986-326 |
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683-986-335 |
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676-944-324 |
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10 ml |
EUR 388.8 |
Cellufine Formyl |
676-944-335 |
JNC America |
10 lt |
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676-943-324 |
JNC America |
10 ml |
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Cellufine Sulfate |
19849 |
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10 lt |
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684-987-335 |
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10 lt |
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685-987-330 |
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5 lt |
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19-546 |
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500 ml |
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21-303 |
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21-605 |
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670000335 |
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21-001 |
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500 ml |
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21-002 |
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21-003 |
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21-904 |
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21-100 |
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21-102 |
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21-103 |
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681-984-324 |
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10 ml |
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Cellufine ETclean L |
681-984-326 |
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50 ml |
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Cellufine ETclean L |
681-984-328 |
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500 ml |
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Cellufine ETclean L |
681-984-330 |
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681-984-335 |
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682-985-324 |
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10 ml |
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Cellufine ETclean S |
682-985-326 |
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50 ml |
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Cellufine ETclean S |
682-985-328 |
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500 ml |
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Cellufine ETclean S |
682-985-330 |
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682-985-335 |
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10 lt |
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20700 |
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100 ml |
EUR 439.2 |
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20702 |
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20703 |
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10 lt |
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Cellufine GCL-2000HF |
21-400 |
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100 ml |
EUR 362.4 |
Cellufine GCL-2000HF |
21-401 |
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500 ml |
EUR 542.4 |
Cellufine GCL-2000HF |
21-402 |
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21-403 |
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20-302 |
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20-303 |
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20-402 |
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20-602 |
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20-603 |
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10 lt |
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21-500 |
JNC America |
50 ml |
EUR 450 |
Cellufine MAX AminoButyl |
21-501 |
JNC America |
100 ml |
EUR 746.4 |
Cellufine MAX AminoButyl |
21-502 |
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500 ml |
EUR 2959.2 |
Cellufine MAX AminoButyl |
21-503 |
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5 lt |
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21-504 |
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10 lt |
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20800 |
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100 ml |
EUR 439.2 |
Cellufine MAX Phenyl LS |
20802 |
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5 lt |
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Cellufine MAX DexS-Hbp |
21-700 |
JNC America |
10 ml |
EUR 312 |
Cellufine MAX DexS-Hbp |
21-700-15 |
JNC America |
1 x 5 ml |
EUR 362.4 |
Cellufine MAX DexS-Hbp |
21-700-51 |
JNC America |
5 x 1 ml |
EUR 362.4 |
Cellufine MAX DexS-Hbp |
21-701 |
JNC America |
50 ml |
EUR 580.8 |
Cellufine MAX DexS-Hbp |
21-702 |
JNC America |
500 ml |
EUR 2882.4 |
Cellufine MAX DexS-Hbp |
21-704 |
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10 lt |
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Cellufine MAX DexS-Vir |
21-800 |
JNC America |
10 ml |
EUR 312 |
Cellufine MAX DexS-Vir |
21-801 |
JNC America |
50 ml |
EUR 580.8 |
Cellufine MAX DexS-Vir |
21-802 |
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500 ml |
EUR 2882.4 |
Cellufine MAX DexS-Vir |
21-804 |
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10 lt |
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Cellufine MAX DexS-Hbp |
21703 |
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5 lt |
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21803 |
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Assay Medium 3A -500 ml |
79816-2 |
BPS Bioscience |
500 ml |
EUR 600 |
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines. • CD155 / TCR Activator - CHO Recombinant Cell Line #60548 • TIGIT / NFAT Reporter - Jurkat Cell Line #60538 |
A-375 Cells Complete Medium |
CM-0014-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-431 Cells Complete Medium |
CM-0015-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-673 Cells Complete Medium |
CM-0017-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-427 Cells Complete Medium |
CM-0253-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-498 Cells Complete Medium |
CM-0254-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-375 Cells Complete Medium |
CM-0014 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-431 Cells Complete Medium |
CM-0015 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-673 Cells Complete Medium |
CM-0017 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-427 Cells Complete Medium |
CM-0253 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-498 Cells Complete Medium |
CM-0254 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-1 Medium |
M874-100G |
EWC Diagnostics |
1 unit |
EUR 20.22 |
Description: A-1 Medium |
C-33 A Cells Complete Medium |
CM-0045-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
C-33 A Cells Complete Medium |
CM-0045 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
HEC-1-A Cells Complete Medium |
CM-0099-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 108 |
Description: Complete Growth Medium |
HEC-1-A Cells Complete Medium |
CM-0099 |
Elabscience Biotech |
125mL×4 |
EUR 108 |
|
Description: Cell lines complete growth medium |
A549 [A-549] Cells Complete Medium |
CM-0016-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 108 |
Description: Complete Growth Medium |
A875 [A-875] Cells Complete Medium |
CM-0255-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 98 |
Description: Complete Growth Medium |
A-204 [A204] Cells Complete Medium |
CM-0475-125mL4 |
Elabscience Biotech |
125 mL×4 |
EUR 108 |
Description: Complete Growth Medium |
A549 [A-549] Cells Complete Medium |
CM-0016 |
Elabscience Biotech |
125mL×4 |
EUR 108 |
|
Description: Cell lines complete growth medium |
A875 [A-875] Cells Complete Medium |
CM-0255 |
Elabscience Biotech |
125mL×4 |
EUR 98 |
|
Description: Cell lines complete growth medium |
A-204 [A204] Cells Complete Medium |
CM-0475 |
Elabscience Biotech |
125mL×4 |
EUR 108 |
|
Description: Cell lines complete growth medium |
Wort Medium w/ Sterile Cellulose |
MF017F-20PT |
EWC Diagnostics |
1 unit |
EUR 37.6 |
Description: Wort Medium w/ Sterile Cellulose |
Wort Medium w/ Sterile Cellulose |
MF017F-50PT |
EWC Diagnostics |
1 unit |
EUR 79.92 |
Description: Wort Medium w/ Sterile Cellulose |
Teepol Medium w/ Sterile Cellulose |
MF015F-20PT |
EWC Diagnostics |
1 unit |
EUR 37.6 |
Description: Teepol Medium w/ Sterile Cellulose |
Nonetheless, co-treatment with TUDCA alleviated inflammatory response induced by IL-1β, as proven by down regulation of Il-1β, Il-6, Il-8 and Cox2, and elevated the expression of antioxidant enzyme Sod2. Moreover, TUDCA enhanced Col IIα expression in IL-1β- and TNC-stimulated cells, however solely in normoxic circumstances. Altogether, these outcomes counsel that though TUDCA could show chondoprotective potential in ER-stressed cells, additional analyses are nonetheless crucial to totally affirm its attainable advice as potential candidate in OA remedy.
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